Crystal structure of a complete ternary complex of T-cell receptor, peptide-MHC, and CD4

  • Introduction
  • Structural Determination
  • Structural Overview
  • MHC-TCR Docking orientation
  • Functional Implications
  • Materials and methods
  • Further Research
  • References

References

List:
  1. Hahn M; Nicholson MJ;Pyrdol J; et al.Source: NATUREIMMUNOLOGY (2005).6(5), 5405-5410  
  2. Yin. Y, Wang. XX & Mariuzza.RA: Crystal structure of a complete ternary complex of T-cell receptor, peptide–MHC, and CD4 (2012)PNAS 2012 109 (14) 5405-5410;
  3. James P. Browne S., Dai S., Gapin L. & Kappler J.W : Annual review of Immunology (2008), (26)
  4. Murphy, M. & Travers, P. (2008) Janeway's immunobiology 7th edn. Garland: Science








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Figure: TCR-pMHC-CD4 complex

Contributors

  • AlbertMaung
  • Vlad
Summary of Side Links

1. Introduction -T cell significance -Objective
2. Structural Determination -Use of stable CD4 mutant -Determining Structure
3. Structural Overview -Angles between structures -CD4 and MHC at concavity -No contact between TCR and CD4 -Comparing bound and unbound
4. MHC-TCR Docking orientation - Orientation - Evolutionary explanations
5. Functional Implications - CD3 location - Possible dimerization
6. Materials and Methods -Protein Production and Purfication -SPR Analysis -Crystallization and Data Collection -Structure Determination and Refinement
7. Further research
8. References







This blog article is mainly based on the article "Crystal structure of a complete ternary complex of T-Cell receptor, peptide-MHC and CD4'' PNAS April 3, 2012 vol. 109 no. 14 5405-5410

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